ABSTRACT
Objective: To establish the preparation technology of propolis extract. Methods: With the contents of total flavonoids, chrysin, pinocembrin, and galangin and extract yield as evaluation indexes, the extraction methods, extraction solvent, extraction times, extraction time, and amount of solvent extraction were optimized with single factor test and orthogonal design to investigate the effect on the preparation technology of propolis extract. Results: The optimal extraction technology was that propolis was refrigerated, grinded, soaked with 95% five times the amount of ethanol, shaked, then extracted for 4 h, filtrated, concentrated, and dried after ethanol recovery. Conclusion: The optimial extracting technology of propolis is stable and feasible, and suitable for further application.